Histological dating endometrium

15-Dec-2019 00:16 by 4 Comments

Histological dating endometrium

Also it has been reported that a defective luteal phase should be suspected only when endometrial histology lags at least 3 d behind the prospective chronological dating of the biopsy (8).Finally, our sequential study of endometrial biopsy specimens in infertile patients showed that at least two endometrial biopsies from separate cycles are needed to evaluate endometrial function, and a third biopsy must be taken in patients with divergent findings in the first two endometrial biopsies (9).

If the biopsy is obtained as close to the expected menses as possible, almost the entire steroidogenic function of the corpus luteum is reflected in the endometrial histological pattern.

All endometrial samples were obtained on postovulatory d 7 as determined by ultrasonography.

Agreement between the repeated observations (first third biopsies) with regard to histological dating and the presence or absence of αvβ3 integrin and pinopods in the endometrium was analyzed using the 2 × 2 frequency tables and Cohen’s κ coefficient.

A NORMAL ENDOMETRIAL milieu is essential in embryo implantation, and understanding the factors that contribute to a receptive endometrium is, at present, a pivotal area of research.

Traditionally, this has been accomplished by histological dating of the endometrial biopsy specimen obtained in the late secretory phase (1, 2).

Despite those attempts to refine the technique of endometrial biopsy, the definitive study to validate this diagnostic approach has never been done, and thus the relationship between histological changes and endometrial receptivity remains unknown (3, 10, 11).

In recent years, an intensive search for specific markers for receptivity has been undertaken.It has been proposed that the in phase endometrium may exhibit aberrant behavior, not correlated with histological delay, yet still be associated with decreased cycle fecundity (11).Markers of normal endometrial development are being uncovered that, according to some authors, will allow us to go beyond merely histological criteria in the evaluation of endometrial function and receptivity.The κ values ranged from a low of −0.39 (level of agreement, poor) for αvβ3 integrin expression to a high of 0.32 (level of agreement, fair) for biopsy dating by anticipated window of implantation.Overall, these results demonstrate that all endometrial variables investigated had poor reproducibility and high variability cycle to cycle.The histologic features of what constitutes “normal” endometrium change with a woman’s age, through the premenarchal, reproductive, perimenopausal, and postmenopausal years.